Analysis Note

ControlPOSITIVE CONTROL:

IMR-5 cells, brain or retina.

Application

Immunoblotting: 1 µg/mL

Immunocytochemistry

Immunohistochemistry (frozen and formalin/paraffin): 1-2 µg/mL. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

Optimal working dilutions must be determined by end user.

Research CategoryNeuroscience

Research Sub CategorySensory & PNS

Detect Rhodopsin using this Anti-Rhodopsin Antibody, clone RET-P1 validated for use in IC & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Membrane preparation from adult rat retina.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Purified immunoglobulin. Liquid in 10 mM PBS, pH 7.4 with 0.2% BSA and 15 mM sodium azide.

Specificity

Reacts with a protein of 39 kDa identified as rhodopsin (opsin). MAB5316 specifically labels the axons and synaptic pedicles of the rods.

CELLULAR LOCALIZATION:

Cytoplasmic

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months.